《植物生理学报》 2012, 48(11): 1113-1119

基因型与培养条件对羽衣甘蓝小孢子胚胎发生的影响

戴希刚^1,2, 施雪萍¹, 包满珠^1,*

1华中农业大学园艺林学学院, 武汉430070; 2江汉大学生命科学学院, 武汉430056

通信作者：包满珠；E-mail: mzbao@mail.hzau.edu.cn；Tel: 027-87282435

摘　要：

本文详细地研究了小孢子发育时期、基因型与培养条件对羽衣甘蓝小孢子胚胎发生的影响, 建立了一个稳定、高频 地获得小孢子胚胎的有效体系。结果表明, 不同基因型材料相同大小的花蕾其小孢子发育时期存在很大差异, 需针对不同 基因型材料选取适合大小的花蕾。供试的37个基因型中, 有20个获得了胚状体, 占供试材料的54.1%, 其中基因型‘桃舞’获 得了最高的出胚率, 为123.6个•皿-1。自交系的出胚率比商业品种和F 代杂种的出胚率要低得多, 且自交代数越高, 小孢子 的胚胎发生能力就越弱。在热激培养48 h后加液培养对小孢子的发育能起到积极作用, 向培养基中添加激素和活性炭对小 孢子的胚胎发生无促进作用。

关键词：羽衣甘蓝; 小孢子培养; 胚胎发生; 基因型; 培养条件

收稿：2012-10-19   修定：2012-11-07

资助：农业部“948”项目(2003-Z36)。

Effects of Genotype and Culture Condition on Microspore Embryogenesis of Ornamental Kale (Brassica oleracea var. acephala)

DAI Xi-Gang^1,2, SHI Xue-Ping¹, BAO Man-Zhu^1,*

¹College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; ²College of Life Sciences, Jianghan University, Wuhan 430056, China

Corresponding author: BAO Man-Zhu; E-mail: mzbao@mail.hzau.edu.cn; Tel: 027-87282435

Abstract:

We have developed an efficient and reliable protocol for deriving microspore embryogenesis in ornamental kale by evaluating the effects of genotype and culture condition on microspore embryogenesis. There were significant differences on the microspore development stage in the same size buds of different genotypes. So the suitable size buds should be selected in view of the different genotypes. Of all the 37 genotypes, 20 genotypes (54.1%) produced embryos. The genotype ‘Peachy Dancing’ showed the highest embryogenesis frequency with yield of 123.6 embryos per dish. The embryogenesis frequency of inbred lines was lower than that of commercial cultivar and F₁ hybrid, and the embryo yield decreased with increased generation of inbreeding. Medium addition enhanced embryo yield, and additions of plant growth regulator, active charcoal or colchicines into NLN medium had no effect on microspore embryogenesis.

Key words: ornamental kale; microspore culture; embryogenesis; genotype; culture condition